Girltrash: All Night Long is set during one long night in the LGBT sub-culture of Los Angeles. Daisy and Tyler, two rock and roll musicians, are selected to partake in a battle of the bands contest to claim a prize.
However, their plans are derailed when their van breaks down and they are joined by Daisy's younger sister, Colby, a recent college graduate. Colby agrees to drive them to the concert if they help her hook up with her girl-crush, a struggling actress named Misty.
Accompanying Misty is her bisexual best friend Sid, who is eager to hook up with a famous celebrity. Misty, however, is more interested in Tyler than Colby. Meanwhile, Daisy is trying to win back her ex-girlfriend, Xan, who is on a date with her latest girlfriend - who happens to be competing against Daisy and Tyler's band that very night.
Elsewhere, Monique Jones, a violent ex-con recently paroled from prison, is on the hunt for Daisy and Tyler with a score to settle.