Desmond Miles returns to Renaissance Italy to follow the life of his ancestor Ezio Auditore. After seeing his home destroyed, Ezio travels to Rome in an effort to rebuild the Assassin Order and bring down the Borgia family, who rule the city with an iron fist. Along the way, the modern day assassins hope to discover the location of the Piece of Eden, or "The Apple" before the Templars find out first.